Rp hplc method for estimation of ferrous ascorbate

Bleomycin is the key ingredient of the assay and so the value of NTBI determined by this technique is frequently addressed as BDI [ 10 ].

ROS are known to damage cellular membranes by inducing lipid peroxidation, damage DNA, proteins and lipids [ 4 ]. BDI has been detected in various biological fluids like serum from patients of idiopathic hemochromatosis, thalassemia major, hematological malignancies after chemotherapy, patients with end stage renal disease, in overt diabetes and in acute coronary syndrome [ 1028535758 ].

Direct estimation of NTBI with iron sensitive fluorescent probe. Iron only in ferrous form can catalyze such reaction and to facilitate the reaction all the ferric ions were converted to ferrous form by using reducing agent like ascorbate which further advances the reaction and degrades the DNA present in the reaction mixture.

Another drawback of this method is its indirect way of detection. In the method described by Gosriwatana et al. Methanol was used as the blank. The percent inhibition was calculated as follows: This approach should be further studied for its elaborated application as the assay is simple, quick and requires least specialized instruments and accessories.

This step is operated to minimize the donation of chelated iron to the free sites of traditional iron binding protein like apotransferrin aTf. The roots were washed thoroughly with distilled water to remove the adhering sand particles and shade dried. The oxygen metabolites including free radical species, superoxide, hydroxyl radicals and other metabolites like hydrogen peroxide and hypohalous acid are often been collectively called as reactive oxygen metabolites ROMor ROS or simply as oxidants.

The observed effects might be due to the high content of polyphenols, A good number of methods experimented by various researchers, nevertheless their comparability is questioned due to lack of reliable inter laboratory analysis.

It has been observed that with the increase in the concentration of NTA there is also a parallel increase in the concentration of NTBI estimated [ 63 ].

The assay was based on fluorescent labeled apo transferrin Fl-aTf which undergoes for fluorescent quenching when it binds to iron.

After incubation for half an hour, AAPH mMa peroxyl radical inducer, was added to all of the wells, except the control, and incubated for 30 min.

The plant was identified by Dr. All such techniques have their own advantages and disadvantages. Nardostachys jatamansi DC is a member of the plant family, Valerianaceae, is a perennial herb growing to about 10—60 m in height and is commonly referred to as Indian spikenard.

The procedure includes heating step for production of end point colored complex [ thiobarbituric acid 2-malondialdehyde] which add some more negative views in the methodology [ 56 ]. The similar method with some modification have been experimented and successfully compared with electro thermal atomic absorption spectroscopy [ 68 ].

Pathogenesis Serum iron if it is not guarded by the Tf, the unbound iron NTBI poses a great challenge to the protective mechanism of the body.

To maintain specific pH usage of buffer is imperative, however much care should be taken as it can introduce artifactual iron to the reaction mixture and generate misleading results.

Media were changed every alternate day, and the experiment was carried out in serum-free media. Experimental cell culture studies had shown that addition of NTBI to human endothelial cell culture increase the surface expression of adhesion molecules and also increase the monocyte adherence to endothelium [ 9596 ].

Consumption of natural antioxidants is associated with reduced risks of cancer, cardiovascular disease, diabetes and other diseases associated with ageing [ 89 ]. One milliliter of 10 mM DNPH dinitrophenyl hydrazine in 2 N HCl was added to the reaction mixture 2 mg of proteinand samples were incubated for 1 h at room temperature.

In recent years, consumption of the natural phytochemicals present in berry crops, teas, herbs, oilseeds, beans, fruits and vegetables have markedly increased [ 1011 ].

Iron induced oxidative damage has also been linked to increased cardiomyocyte loss due to apoptosis [ 93 ]. The eluting compounds were detected by monitoring at nm.

Statistical Analysis All of the analyses were carried out in triplicate. However Kolb et al. The other chemicals and reagents were of analytical grade and were procured from Merck, Bangalore, India.• Researched “Development of Stability Indicating HPTLC Method for Simultaneous Estimation of Telmisartan and Indapamide in Bulk and Pharmaceutical Dosage Form” and published in • Operated HPTLC, HPLC, UV-VIS spectrophotometer, spectrofluorometer, flame photometer, colorimeter, dissolution apparatus with proficiency in instrument Title: Seeking job in Retail Pharmacy.

development and validation of a rapid rp-hplc method for the estimation of ziprasidone hydrochloride monohydrate in drug substance and its dosage forms GANJI RAMANAIAH, DR.


Non Transferrin Bound Iron: Nature, Manifestations and Analytical Approaches for Estimation

RP-HPLC method for estimation ferrous ascorbate and folic acid in bulk and in pharmaceutical formulation was successfully developed as per ICH guidelines. The method was found accurate, precise and sensetive hence it can be used for routine analysis by any analyst.

Another important aspect is that the ascorbate which is used as reducing agent may not convert all the ferric irons to ferrous iron and can lead to false low results. The method also gets influenced by hemoglobin and applicable only when Tf saturation is >80 % [ 10, 55 ].

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67th APTI Conference procedings. Download. 67th APTI Conference procedings. Uploaded by. Antioxidant, Biomolecule Oxidation Protective Activities of Nardostachys jatamansi DC and Its Phytochemical Analysis by RP-HPLC and GC-MS Sakina Razack, 1 Kandikattu Hemanth Kumar, 1 Ilaiyaraja Nallamuthu, 1 Mahadeva Naika, 2 and Farhath Khanum 1, *.

Rp hplc method for estimation of ferrous ascorbate
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